Anticorps Polyclonal de lapin anti-CDC5L

CDC5L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 12974-1-AP

Synonymes

Cdc5 like protein, CDC5L, CEF1, hCDC5, KIAA0432, PCDC5RP, Pombe cdc5 related protein

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Galerie de données de validation

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  WB analysis of human brain using 12974-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12974-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12974-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12974-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12974-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12974-1-AP

  IP result of anti-CDC5L(IP:12974-1-AP, 4ug; Detection:12974-1-AP 1:500) with mouse brain tissue lysate 1600 ug.

• 

  IHC staining of mouse testis using 12974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12974-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 12974-1-AP (CDC5L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HEK-293, cellules HepG2, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

12974-1-AP cible CDC5L dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDC5L Protéine recombinante Ag3578
• Nom complet: CDC5 cell division cycle 5-like (S. pombe)
• Masse moléculaire calculée: 802 aa, 92 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC001568
• Symbole du gène: CDC5L
• Identification du gène (NCBI): 988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
	WB	J Biol Chem; O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.; Authors - Li Liu
human	WB	Cancer Med; Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway.; Authors - Chunmei Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wael (Verified Customer) (01-19-2023)

CDC5L detected in both nuclear and cytoplasmic fractions of Hek293 lysates. specific band between 100 and 130kd.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hek293

CDC5L Antibody Western Blot validation (1:2000 dilution) in Hek293 (Cat no:12974-1-AP)