Anticorps Recombinant de lapin anti-CDC5L

CDC5L Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250089G5

N° de cat : 85788-5-PBS

Synonymes

CDC5, Cdc5-like protein, CEF1, Cell division cycle 5-like protein, KIAA0432

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Galerie de données de validation

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  WB analysis using 85788-5-RR (same clone as 85788-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85788-5-RR (CDC5L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85788-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85788-5-RR (same clone as 85788-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (85788-5-RR, Clone: 250089G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85788-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85788-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85788-5-RR against Human CDC5L were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85788-5-PBS cible CDC5L dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDC5L Protéine recombinante Ag3578
• Nom complet: CDC5 cell division cycle 5-like (S. pombe)
• Masse moléculaire calculée: 802 aa, 92 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC001568
• Symbole du gène: CDC5L
• Identification du gène (NCBI): 988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDC5L is ubiquitously expressed in humans, with relatively high expression levels in testis, bone marrow, and 25 other tissues. In proliferating cells, CDC5L is constitutively expressed, suggesting its regulation may occur through post-translational modifications. CDC5L plays a critical role in the cell cycle, particularly in the G2/M transition. It interacts with cyclins and cyclin-dependent kinases (CDKs), helping to control the timing and accuracy of cell division.

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