Anticorps Polyclonal de lapin anti-CDCA2

• Phare
• Validé par KD/KO

CDCA2 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17701-1-AP

Synonymes

CDCA2, Repo Man

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Galerie de données de validation

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  WB analysis of Jurkat using 17701-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17701-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17701-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17701-1-AP

  IP Result of anti-CDCA2 (IP:17701-1-AP, 4ug; Detection:17701-1-AP 1:800) with Jurkat cells lysate 2800ug.

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  IF Staining of HepG2 using 17701-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17701-1-AP (CDCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, cellules MCF-7
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

17701-1-AP cible CDCA2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDCA2 Protéine recombinante Ag11967
• Nom complet: cell division cycle associated 2
• Masse moléculaire calculée: 863 aa, 95 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC036214
• Symbole du gène: CDCA2
• Identification du gène (NCBI): 157313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell division cycle associated 2(CDCA2) is a nuclear protein that is a specific regulatory subunit for protein phosphatase 1γ (PP1γ). CDCA2 is a candidate factor involved in preparing mitotic chromatin for the transition to interphase, and controls PP1γ-dependent essential DDR regulationCDCA2 regulates the DNA damage response(DDR) in cell cycle. In addition, CDCA2 modulates phosphorylation of major mitotic histone H3 in a PP1-dependent manner. We got 125 kDa in western blotting maybe due to its phosphorylation.

Publications

Species	Application	Title
human	WB	Oncogene; CDCA2 protects against oxidative stress by promoting BRCA1-NRF2 signaling in hepatocellular carcinoma.; Authors - Shouping Wang ; KD Validated
human	WB	Oncogene; MCM8 is regulated by EGFR signaling and promotes the growth of glioma stem cells through its interaction with DNA-replication-initiating factors.; Authors - Xiaoliang Wang
human	IF	Sci Rep; Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.; Authors - Honghai Xia
human	WB,IF,IP	J Cell Sci; SUMOylation of RepoMan during late telophase regulates dephosphorylation of lamin A.; Authors - Takanobu Moriuchi ; KD Validated
human	WB,IHC	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen