Anticorps Polyclonal de lapin anti-CDCA2

• Phare
• Validé par KD/KO

CDCA2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17701-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 17701-1-AP (same clone as 17701-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 17701-1-AP (same clone as 17701-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 17701-1-AP (same clone as 17701-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 17701-1-AP (same clone as 17701-1-PBS)

  IP result of anti-CDCA2 (IP:17701-1-AP, 4ug; Detection:17701-1-AP 1:800) with Jurkat cells lysate 2800ug. This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17701-1-AP (same clone as 17701-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17701-1-AP (CDCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17701-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17701-1-PBS cible CDCA2 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDCA2 Protéine recombinante Ag11967
• Nom complet: cell division cycle associated 2
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC036214
• Symbole du gène: CDCA2
• Identification du gène (NCBI): 157313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cell division cycle associated 2(CDCA2) is a nuclear protein that is a specific regulatory subunit for protein phosphatase 1γ (PP1γ). CDCA2 is a candidate factor involved in preparing mitotic chromatin for the transition to interphase, and controls PP1γ-dependent essential DDR regulationCDCA2 regulates the DNA damage response(DDR) in cell cycle. In addition, CDCA2 modulates phosphorylation of major mitotic histone H3 in a PP1-dependent manner. We got 125 kDa in western blotting maybe due to its phosphorylation.

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