Anticorps Recombinant de lapin anti-CDCA2

CDCA2 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250980D8

N° de cat : 86379-1-PBS

Synonymes

Cell division cycle-associated protein 2, Repo Man, Repo-Man

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Galerie de données de validation

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  WB analysis using 86379-1-RR (same clone as 86379-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86379-1-RR (CDCA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86379-1-RR (same clone as 86379-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDCA2 antibody (86379-1-RR, Clone: 250980D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86379-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86379-1-RR against Human CDCA2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86379-1-PBS cible CDCA2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDCA2 Protéine recombinante Ag11967
• Nom complet: cell division cycle associated 2
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC036214
• Symbole du gène: CDCA2
• Identification du gène (NCBI): 157313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cell division cycle associated 2(CDCA2) is a nuclear protein that is a specific regulatory subunit for protein phosphatase 1γ (PP1γ). CDCA2 is a candidate factor involved in preparing mitotic chromatin for the transition to interphase, and controls PP1γ-dependent essential DDR regulationCDCA2 regulates the DNA damage response(DDR) in cell cycle. In addition, CDCA2 modulates phosphorylation of major mitotic histone H3 in a PP1-dependent manner.

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