Anticorps Polyclonal de lapin anti-CDCA7

• Phare
• Validé par KD/KO

CDCA7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15249-1-PBS

Synonymes

Protein JPO1, JPO1, Cell division cycle-associated protein 7

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Galerie de données de validation

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  WB analysis using 15249-1-AP (same clone as 15249-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 15249-1-AP (CDCA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15249-1-AP (same clone as 15249-1-PBS)

  WB result of CDCA7 antibody (15249-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDCA7 transfected HeLa cells. This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15249-1-AP (same clone as 15249-1-PBS)

  IP result of anti-CDCA7 (IP:15249-1-AP, 4ug; Detection:15249-1-AP 1:2000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15249-1-AP (same clone as 15249-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15249-1-AP (CDCA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15249-1-AP (same clone as 15249-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15249-1-AP (CDCA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15249-1-AP (same clone as 15249-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 15249-1-AP (CDCA7 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 15249-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15249-1-PBS cible CDCA7 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDCA7 Protéine recombinante Ag4297
• Nom complet: cell division cycle associated 7
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-43 kDa
• Numéro d’acquisition GenBank: BC027966
• Symbole du gène: CDCA7
• Identification du gène (NCBI): 83879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDCA7, also named as JP01, participates in MYC-mediated cell transformation and apoptosis. It plays a role as transcriptional regulator.

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