Anticorps Monoclonal anti-CDCA8

CDCA8 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A10C9

N° de cat : 68510-1-PBS

Synonymes

2A10C9, BOR, Borealin, Cell division cycle-associated protein 8, Dasra B

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Galerie de données de validation

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  WB analysis using 68510-1-Ig (same clone as 68510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68510-1-Ig (CDCA8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 68510-1-Ig (same clone as 68510-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68510-1-Ig (same clone as 68510-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CDCA8 antibody (68510-1-Ig, Clone: 2A10C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68510-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68510-1-PBS cible CDCA8 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDCA8 Protéine recombinante Ag3136
• Nom complet: cell division cycle associated 8
• Masse moléculaire calculée: 280 aa, 31 kDa
• Poids moléculaire observé: 31-38 kDa
• Numéro d’acquisition GenBank: BC016944
• Symbole du gène: CDCA8
• Identification du gène (NCBI): 55143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The predicted molecular weight of CDCA8 is about 31 kDa. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.

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