Anticorps Polyclonal de lapin anti-P-cadherin

P-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 13773-1-AP

Synonymes

Cadherin 3, CDH3, CDHP, HJMD, PCAD, P-cadherin

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Galerie de données de validation

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  WB analysis of A431 using 13773-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13773-1-AP (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 13773-1-AP

  IP Result of anti-P-cadherin (IP:13773-1-AP, 4ug; Detection:13773-1-AP 1:300) with A431 cells lysate 3040ug.

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  IHC staining of human breast cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 13773-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A431 using 13773-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 13773-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 13773-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human P-cadherin (13773-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431,
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

13773-1-AP cible P-cadherin dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P-cadherin Protéine recombinante Ag4758
• Nom complet: cadherin 3, type 1, P-cadherin (placental)
• Masse moléculaire calculée: 829 aa, 91 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC041846
• Symbole du gène: CDH3
• Identification du gène (NCBI): 1001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.; Authors - Hans I-Chen Harn
mouse	IF	Mol Ther Nucleic Acids; Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.; Authors - Ziyang Wang
human	IHC	Aging (Albany NY); Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.; Authors - Jing Han
human	WB,IHC	Front Oncol; Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer.; Authors - Meng-Jie Shan
human	WB,IHC	Int J Biol Sci; KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling.; Authors - Li Li
mouse	IF	Dev Dyn; Denticleless E3 ubiquitin protein ligase (DTL) maintains the proliferation and differentiation of epidermis and hair follicles during skin development; Authors - Yanhui Lin