Anticorps Monoclonal anti-VE-cadherin/CD144

VE-cadherin/CD144 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(43)

CloneNo.

4F9B10

N° de cat : 66804-1-Ig

Synonymes

VE-cadherin, 4F9B10, 7B4, 7B4 antigen, Cadherin 5

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Galerie de données de validation

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  WB analysis of human placenta using 66804-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66804-1-Ig (VE-cadherin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66804-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66804-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66804-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66804-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66804-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66804-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 66804-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using VE-cadherin antibody (66804-1-Ig, Clone: 4F9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HUVEC using 66804-1-Ig

  Immunofluorescent analysis of un-fixed HUVEC cells using VE-cadherin/CD144 antibody (66804-1-Ig, Clone: 4F9B10 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'amygdalite humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu placentaire humain,
• Résultats positifs en IF/ICC: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 25 publications below
• IHC: See 4 publications below
• IF: See 10 publications below

Informations sur le produit

66804-1-Ig cible VE-cadherin/CD144 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VE-cadherin/CD144 Protéine recombinante Ag27501
• Nom complet: cadherin 5, type 2 (vascular endothelium)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 125 kDa, 100 kDa
• Numéro d’acquisition GenBank: NM_001795
• Symbole du gène: VE-cadherin
• Identification du gène (NCBI): 1003
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).

Publications

Species	Application	Title
human	WB	Acta Pharmacol Sin; Endothelial deubiquinatase YOD1 mediates Ang II-induced vascular endothelial-mesenchymal transition and remodeling by regulating β-catenin; Authors - Wan-Te Lin
	WB	EMBO Rep; Mouse endothelial OTUD1 promotes angiotensin II-induced vascular remodeling by deubiquitinating SMAD3; Authors - Zhuqi Huang
		Biomed Pharmacother; Ginsenoside Rg1 ameliorates hypoxia-induced pulmonary arterial hypertension by inhibiting endothelial-to-mesenchymal transition and inflammation by regulating CCN1; Authors - Bai-Lin Tang
human	IF	Regen Biomater; Microvascular network based on the Hilbert curve for nutrient transport in thick tissue; Authors - Zhenxing Wang
human	IF	J Invest Dermatol; Endothelial Piezo1 mediates barrier dysfunction and NLRP3 inflammasomes activation in psoriasis; Authors - Lixin Yue
human	IF	J Cell Mol Med; Adrenomedullin Inhibits the Efficacy of Combined Immunotherapy and Targeted Therapy in Biliary Tract Cancer by Disrupting Endothelial Cell Functions; Authors - Zhengfeng Xuan

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