Anticorps Monoclonal anti-CDK1

CDK1 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

2A11E4

N° de cat : 67575-1-PBS

Synonymes

CDC2, 2A11E4, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

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Galerie de données de validation

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  WB analysis using 67575-1-Ig (same clone as 67575-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67575-1-Ig (CDK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67575-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67575-1-Ig (same clone as 67575-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK1 Monoclonal antibody (67575-1-Ig, Clone:2A11E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67575-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67575-1-PBS cible CDK1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDK1 Protéine recombinante Ag29018
• Nom complet: cell division cycle 2, G1 to S and G2 to M
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC014563
• Symbole du gène: CDK1
• Identification du gène (NCBI): 983
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

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