Anticorps Polyclonal de lapin anti-CDK13

CDK13 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30461-1-PBS

Synonymes

CDC2L5, CHED, CDC2L, CDC2 related protein kinase 5

---

Galerie de données de validation

• 

  WB analysis using 30461-1-AP (same clone as 30461-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30461-1-AP (CDK13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 30461-1-AP (same clone as 30461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30461-1-AP (CDK13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 30461-1-AP (same clone as 30461-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK13 antibody (30461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 30461-1-AP (same clone as 30461-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDK13 (30461-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 30461-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

30461-1-PBS cible CDK13 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK13 Protéine recombinante Ag32897
• Nom complet: cell division cycle 2-like 5 (cholinesterase-related cell division controller)
• Masse moléculaire calculée: 165 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: NM_003718.4
• Symbole du gène: CDK13
• Identification du gène (NCBI): 8621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin-dependent kinase 13 (CDK13) is also named as CDC2L, CDC2L5, CHED and KIAA1791, and belongs to the CMGC Ser/Thr protein kinase family. Interestingly, in TNBC cells silencing CDK13 provokes cell death without affecting DDR genes. Like CDK12, CDK13 phosphorylates the Ser2 position of the heptad repeat in the CTD of RNA Pol II (PMID:26748711). CDK13/CycK complex is that regulates transcription by phosphorylating serine residues at positions 5 and 2 of the heptapeptide repeats (Y-S-P-T-S-P-S) comprising the C-terminal domain (CTD) of RPB1, the largest subunit of RNA polymerase II (RNAPII). CDK13 is expressed in fetal brain, liver, and muscle, and adult whole brain, hippocampus, and bone marrow (PMID:30904094).

{{ptg:RelatedPrimaryAntibodies}}