Anticorps Monoclonal anti-CDK2

CDK2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

4E2E10

N° de cat : 60312-1-PBS

Synonymes

4E2E10, CDKN2, Cell division protein kinase 2, Cyclin dependent kinase 2, Cyclin-dependent kinase 2

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Galerie de données de validation

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  WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60312-1-Ig (CDK2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60312-1-Ig (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60312-1-Ig (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (60312-1-Ig, Clone: 4E2E10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 60312-1-Ig (same clone as 60312-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CDK2 Monoclonal antibody (60312-1-Ig, Clone:4E2E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60312-1-PBS cible CDK2 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDK2 Protéine recombinante Ag21310
• Nom complet: cyclin-dependent kinase 2
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC003065
• Symbole du gène: CDK2
• Identification du gène (NCBI): 1017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

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