Anticorps Recombinant de lapin anti-CDK2

CDK2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240580A11

N° de cat : 83635-4-PBS

Synonymes

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

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Galerie de données de validation

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  WB analysis using 83635-4-RR (same clone as 83635-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83635-4-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83635-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83635-4-RR (same clone as 83635-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-4-RR, Clone:240580A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83635-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83635-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83635-4-RR against Human CDK2 were performed. The affinity constant is 8.32 nM.

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Informations sur le produit

83635-4-PBS cible CDK2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDK2 Protéine recombinante Ag17133
• Nom complet: cyclin-dependent kinase 2
• Masse moléculaire calculée: 298 aa, 34 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC003065
• Symbole du gène: CDK2
• Identification du gène (NCBI): 1017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

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