Anticorps Polyclonal de lapin anti-CDK2AP1

CDK2AP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 13060-2-AP

Synonymes

CDK2 associated protein 1, CDK2-associated protein 1, CDKAP1, Cyclin-dependent kinase 2-associated protein 1, Deleted in oral cancer 1

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Galerie de données de validation

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  WB analysis of HEK-293 using 13060-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13060-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13060-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13060-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 13060-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13060-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2AP1 antibody (13060-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu placentaire humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

13060-2-AP cible CDK2AP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK2AP1 Protéine recombinante Ag3736
• Nom complet: cyclin-dependent kinase 2 associated protein 1
• Masse moléculaire calculée: 115 aa, 13 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC034717
• Symbole du gène: CDK2AP1
• Identification du gène (NCBI): 8099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Oncol Lett; Cathepsin L interacts with CDK2-AP1 as a potential predictor of prognosis in patients with breast cancer.; Authors - Zhan Wang

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