Anticorps Recombinant de lapin anti-CDK4

• Phare
• Validé par KD/KO

CDK4 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241053F10

N° de cat : 83994-3-PBS

Synonymes

241053F10, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, Cyclin-dependent kinase 4

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Galerie de données de validation

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  WB analysis using 83994-3-RR (same clone as 83994-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83994-3-RR (CDK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83994-3-RR (same clone as 83994-3-PBS)

  WB result of CDK4 antibody (83994-3-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK4 transfected HeLa cells. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 83994-3-RR (same clone as 83994-3-PBS)

  IP result of anti-CDK4 (IP:83994-3-RR, 4ug; Detection:83994-3-RR 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 83994-3-RR (same clone as 83994-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83994-3-RR (CDK4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83994-3-RR (same clone as 83994-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (83994-3-RR, Clone: 241053F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

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Informations sur le produit

83994-3-PBS cible CDK4 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDK4 Protéine recombinante Ag20538
• Nom complet: cyclin-dependent kinase 4
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC010153
• Symbole du gène: CDK4
• Identification du gène (NCBI): 1019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1-to S-phase transition during the cell cycle, and its expression is primarily controlled at the transcriptional level(PMID:17253961). The CCND1-CDK4 axis is critical not only in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment, which are vital to sustain tumor outgrowth (PMID:21844184).

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