Anticorps Monoclonal anti-CDK5

CDK5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B9E1

N° de cat : 68514-1-PBS

Synonymes

1B9E1, Cdk 5, Cyclin-dependent kinase 5, Cyclin-dependent-like kinase 5, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 68514-1-Ig (same clone as 68514-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68514-1-Ig (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68514-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68514-1-Ig (same clone as 68514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68514-1-Ig (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68514-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68514-1-Ig (same clone as 68514-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68514-1-Ig (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68514-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 68514-1-Ig (same clone as 68514-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (68514-1-Ig, Clone: 1B9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68514-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68514-1-PBS cible CDK5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDK5 Protéine recombinante Ag28465
• Nom complet: cyclin-dependent kinase 5
• Masse moléculaire calculée: 31 kDa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC005115
• Symbole du gène: CDK5
• Identification du gène (NCBI): 1020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

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