Anticorps Recombinant de lapin anti-CDK5

CDK5 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241908F10

N° de cat : 84749-1-PBS

Synonymes

241908F10, Cdk 5, Cyclin-dependent kinase 5, Cyclin-dependent-like kinase 5, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 84749-1-RR (same clone as 84749-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84749-1-RR (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 84749-1-RR (same clone as 84749-1-PBS)

  IP result of anti-CDK5 (IP:84749-1-RR, 4ug; Detection:84749-1-RR 1:8000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 84749-1-RR (same clone as 84749-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 84749-1-RR (same clone as 84749-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84749-1-RR (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 84749-1-RR (same clone as 84749-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (84749-1-RR, Clone: 241908F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84749-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84749-1-PBS cible CDK5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDK5 Protéine recombinante Ag28465
• Nom complet: cyclin-dependent kinase 5
• Masse moléculaire calculée: 31 kDa, 33 kDa
• Poids moléculaire observé: 31-33 kDa
• Numéro d’acquisition GenBank: BC005115
• Symbole du gène: CDK5
• Identification du gène (NCBI): 1020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.

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