Anticorps Monoclonal anti-CDK5R1

CDK5R1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1A3A5

N° de cat : 67503-1-Ig

Synonymes

CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit



Applications testées

Résultats positifs en WBtissu de cervelet de porc, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de souris
Résultats positifs en IHCtissu cérébral de souris, tissu cérébral de rat
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

67503-1-Ig cible CDK5R1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

Réactivité Humain, Lapin, porc, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène CDK5R1 Protéine recombinante Ag13152
Nom complet cyclin-dependent kinase 5, regulatory subunit 1 (p35)
Masse moléculaire calculée 34 kDa
Poids moléculaire observé 34 kDa
Numéro d’acquisition GenBankBC020580
Symbole du gène CDK5R1
Identification du gène (NCBI) 8851
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.

Protocole

Product Specific Protocols
WB protocol for CDK5R1 antibody 67503-1-IgDownload protocol
IHC protocol for CDK5R1 antibody 67503-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

GRAMD1B is a regulator of lipid homeostasis, autophagic flux and phosphorylated tau

Authors - Diana Acosta Ingram
humanWB,IF,CoIP

J Pharm Anal

Histone deacetylase inhibitor pracinostat suppresses colorectal cancer by inducing CDK5-Drp1 signaling-mediated peripheral mitofission

Authors - Xiao-Ling Liang
mouseIHC

Mol Ther Nucleic Acids

Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease.

Authors - Li Zeng
humanWB

Cancer Lett

CDK5RAP3 is a novel super-enhancer-driven gene activated by master TFs and regulates ER-Phagy in neuroblastoma

Authors - Ran Zhuo
WB

Sci China Life Sci

FSD1 inhibits glioblastoma diffuse infiltration through restriction of HDAC6-mediated microtubule deacetylation

Authors - Dake Xiao
humanWB

Acta Pharm Sin B

CDK5-triggered G6PD phosphorylation at threonine 91 facilitating redox homeostasis reveals a vulnerability in breast cancer

Authors - Yuncheng Bei

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jia Kang (Verified Customer) (09-05-2025)

The antibody worked rather well, providing clear images under the microscope. No particular issue with this specific antibody.

  • Applications: Immunofluorescence
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