Anticorps Monoclonal anti-CDK5R1

CDK5R1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1A3A5

N° de cat : 67503-1-Ig

Synonymes

CDK5 activator 1, CDK5P35, CDK5R, CDK5R1, NCK5A, p23, p25, p35, p35nck5a, TPKII regulatory subunit

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Galerie de données de validation

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  WB analysis using 67503-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of mouse cerebellum using 67503-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67503-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67503-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67503-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67503-1-Ig (CDK5R1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67503-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67503-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67503-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67503-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67503-1-Ig (CDK5R1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de porc, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67503-1-Ig cible CDK5R1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDK5R1 Protéine recombinante Ag13152
• Nom complet: cyclin-dependent kinase 5, regulatory subunit 1 (p35)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC020580
• Symbole du gène: CDK5R1
• Identification du gène (NCBI): 8851
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.

Publications

Species	Application	Title
human	WB,IF,CoIP	J Pharm Anal; Histone deacetylase inhibitor pracinostat suppresses colorectal cancer by inducing CDK5-Drp1 signaling-mediated peripheral mitofission; Authors - Xiao-Ling Liang
mouse	IHC	Mol Ther Nucleic Acids; Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease.; Authors - Li Zeng