Anticorps Monoclonal anti-CDK6

• Phare
• Validé par KD/KO

CDK6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

4B9C11

N° de cat : 66278-1-PBS

Synonymes

4B9C11, CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6

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Galerie de données de validation

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  WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66278-1-Ig (same clone as 66278-1-PBS)

  WB result of CDK6 antibody (66278-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66278-1-Ig (same clone as 66278-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66278-1-Ig (same clone as 66278-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66278-1-Ig (same clone as 66278-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66278-1-Ig (same clone as 66278-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66278-1-Ig (CDK6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66278-1-Ig (same clone as 66278-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66278-1-Ig (CDK6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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  IF Staining of MOLT-4 using 66278-1-Ig (same clone as 66278-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using CDK6 antibody (66278-1-Ig, Clone: 4B9C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66278-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66278-1-PBS cible CDK6 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDK6 Protéine recombinante Ag5600
• Nom complet: cyclin-dependent kinase 6
• Masse moléculaire calculée: 326 aa, 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC027989
• Symbole du gène: CDK6
• Identification du gène (NCBI): 1021
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739). 

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