Anticorps Recombinant de lapin anti-CDK6

CDK6 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241022F11

N° de cat : 84200-1-PBS

Synonymes

241022F11, CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6

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Galerie de données de validation

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  WB analysis using 84200-1-RR (same clone as 84200-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84200-1-RR (CDK6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84200-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84200-1-RR (same clone as 84200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CDK6 antibody (84200-1-RR, Clone: 241022F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84200-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84200-1-RR (same clone as 84200-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK6 antibody (84200-1-RR, Clone: 241022F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84200-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84200-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84200-1-RR against Human CDK6 were performed. The affinity constant is 15.3 nM.

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Informations sur le produit

84200-1-PBS cible CDK6 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDK6 Protéine recombinante Ag5600
• Nom complet: cyclin-dependent kinase 6
• Masse moléculaire calculée: 326 aa, 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC027989
• Symbole du gène: CDK6
• Identification du gène (NCBI): 1021
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739).

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