Anticorps Monoclonal anti-CDK6

• Phare
• Validé par KD/KO

CDK6 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4B9C11

Avis (2)

N° de cat : CL594-66278

Synonymes

4B9C11, CDKN6, Cell division protein kinase 6, Cyclin dependent kinase 6, Cyclin-dependent kinase 6

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Galerie de données de validation

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  IF Staining of HeLa using CL594-66278

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CDK6 antibody (CL594-66278, Clone: 4B9C11 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-66278

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated CDK6 Monoclonal antibody (CL594-66278, Clone: 4B9C11) (red) or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66278 cible CDK6 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDK6 Protéine recombinante Ag5600
• Nom complet: cyclin-dependent kinase 6
• Masse moléculaire calculée: 326 aa, 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC027989
• Symbole du gène: CDK6
• Identification du gène (NCBI): 1021
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739). 

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marian (Verified Customer) (08-26-2025)

Excellent antibody to track cell cycle and dephosphorization or hyper-phosphorylation of pRB using CDK6 expression.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Hematopoietic stem cells

CL594 CDK6 Antibody Flow Cytometry validation (1:250 dilution) in Hematopoietic stem cells (Cat no:CL594-66278)

FH

Marian (Verified Customer) (04-17-2025)

Antibody can be used with BD Cytofix/Cytoperm kit

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Hematopoietic stem cells

CL594 CDK6 Antibody Flow Cytometry validation (1:250 dilution) in Hematopoietic stem cells (Cat no:CL594-66278)

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