Anticorps Polyclonal de lapin anti-CDK7

CDK7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 27027-1-AP

Synonymes

CDKN7, CDK-activating kinase 1, CDK activating kinase, CAK1, CAK

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Galerie de données de validation

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  WB analysis of HepG2 using 27027-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27027-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27027-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 27027-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27027-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27027-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 27027-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK7 antibody (27027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7, tissu embryonnaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

27027-1-AP cible CDK7 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK7 Protéine recombinante Ag25726
• Nom complet: cyclin-dependent kinase 7
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC005298
• Symbole du gène: CDK7
• Identification du gène (NCBI): 1022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	ACS Nano; Nanomaterial-Facilitated Cyclin-Dependent Kinase 7 Inhibition Suppresses Gallbladder Cancer Progression via Targeting Transcriptional Addiction.; Authors - Chen-Song Huang
mouse	WB	Genes Dev; TRF1 uses a noncanonical function of TFIIH to promote telomere replication; Authors - Zhe Yang
human	WB	Front Oncol; Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach; Authors - Xiaobin Fei
human	WB	Cell Signal; TAGLN2 targeted control of ARPC5-mediated activation of the MEK/ERK signaling pathway influences the proliferation, invasion, and metastasis of pancreatic cancer cells; Authors - Yongjia Gao
human,mouse	WB	J Immunother Cancer; Adenovirus vaccine targeting kinases induces potent antitumor immunity in solid tumors; Authors - Fei Zhu
human	WB	Nat Commun; CDK9 recruits HUWE1 to degrade RARα and offers therapeutic opportunities for cutaneous T-cell lymphoma; Authors - Chen-Hui Luo

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