Anticorps Monoclonal anti-CDK7

CDK7 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E7B6

N° de cat : 67889-1-PBS

Synonymes

39 kDa protein kinase, CAK, CAK1, CDK activating kinase, CDK7, CDKN7, Cell division protein kinase 7, cyclin dependent kinase 7, MO15, p39 Mo15, p39MO15, STK1

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Galerie de données de validation

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  WB analysis using 67889-1-Ig (same clone as 67889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  WB analysis using 67889-1-Ig (same clone as 67889-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67889-1-Ig (CDK7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67889-1-Ig (same clone as 67889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67889-1-Ig (same clone as 67889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67889-1-Ig (CDK7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67889-1-Ig (same clone as 67889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67889-1-Ig (same clone as 67889-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CDK7 antibody (67889-1-Ig, Clone: 1E7B6 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67889-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67889-1-PBS cible CDK7 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDK7 Protéine recombinante Ag25737
• Nom complet: cyclin-dependent kinase 7
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC005298
• Symbole du gène: CDK7
• Identification du gène (NCBI): 1022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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