Anticorps Recombinant de lapin anti-CDK8/19

CDK8/19 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250832D1

N° de cat : 86276-1-PBS

Synonymes

CDC2L6, CDC2 related protein kinase 6, CDC2-related protein kinase 6, CDK11, CDK19

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Galerie de données de validation

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  WB analysis using 86276-1-RR (same clone as 86276-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86276-1-RR (CDC2L6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86276-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86276-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86276-1-RR against Human CDK8/19 were performed. The affinity constant is 3.19 nM.

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Informations sur le produit

86276-1-PBS cible CDK8/19 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDK8/19 Protéine recombinante Ag4731
• Nom complet: cell division cycle 2-like 6 (CDK8-like)
• Masse moléculaire calculée: 502 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC037289
• Symbole du gène: CDK19
• Identification du gène (NCBI): 23097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The CDK8 homolog, CDK19 (Cdk11, CDC2L6) was discovered by Hidden Markov model (HMM) profiling of a eukaryotic protein kinase domain, and found to have approximately 91% sequence homology to Cdk8. The two proteins share a particularly high degree of sequence conservation in their kinase and cyclin binding domain (PMID: 30693281, 26985305). CDK8 and CDK19, collectively termed 'Mediator Kinase,' are incorporated, in a mutually exclusive manner, as part of a 4-protein complex called the Mediator kinase module. CDK8/19 inhibition suppressed the induction of NFκB-driven transcription by p21 (PMID: 28855340).

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