Anticorps Polyclonal de lapin anti-CDK9

CDK9 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11705-1-AP

Synonymes

C 2k, CDC2L4, CDK9, Cell division protein kinase 9, CTK1, cyclin dependent kinase 9, PITALRE, TAK

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Galerie de données de validation

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  WB analysis using 11705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11705-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11705-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11705-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11705-1-AP (CDK9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11705-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11705-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11705-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11705-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11705-1-AP (CDK9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (11705-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11705-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CDK9 (11705-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules NIH/3T3, tissu placentaire humain
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

11705-1-AP cible CDK9 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK9 Protéine recombinante Ag2318
• Nom complet: cyclin-dependent kinase 9
• Masse moléculaire calculée: 372 aa, 43 kDa
• Poids moléculaire observé: 38-42 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC001968
• Symbole du gène: CDK9
• Identification du gène (NCBI): 1025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

Publications

Species	Application	Title
human,mouse	WB,IP	Nat Commun; The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.; Authors - Xinzhi Li
mouse	WB	Nat Commun; Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH); Authors - Xinzhi Li
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	Proteomics; A Simplified Thermal Proteome Profiling Approach to Screen Protein Targets of a Ligand.; Authors - Xiaolei Zhang
mouse	IHC,WB	Clin Exp Pharmacol Physiol; Sevoflurane-induced P300 promotes neuron apoptosis via Sp1/SDK9 pathway; Authors - Xiaoqin Zhou
		Chem Biol Drug Des; Synthesis, Structure-Activity Relationship Studies and Biological Evaluation of Novel 2,5-Disubstituted Indole Derivatives as Anticancer Agents.; Authors - Hongyu Hu