Anticorps Monoclonal anti-CDK9

CDK9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G6B3

N° de cat : 67256-1-PBS

Synonymes

1G6B3, C 2k, C-2K, CDC2L4, Cell division cycle 2-like protein kinase 4

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Galerie de données de validation

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  WB analysis using 67256-1-Ig (same clone as 67256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67256-1-Ig (CDK9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67256-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67256-1-Ig (same clone as 67256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (67256-1-Ig, Clone: 1G6B3 ) at dilution of 1:1000 and Multi-rAbCoraLite Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection. This data was developed using the same antibody clone with 67256-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67256-1-PBS cible CDK9 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDK9 Protéine recombinante Ag29178
• Nom complet: cyclin-dependent kinase 9
• Masse moléculaire calculée: 372 aa, 43 kDa
• Poids moléculaire observé: 38-42 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC001968
• Symbole du gène: CDK9
• Identification du gène (NCBI): 1025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

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