Anticorps Recombinant de lapin anti-CDK9

CDK9 Recombinant Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240600D7

N° de cat : 83662-7-PBS

Synonymes

CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K, C 2k

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Galerie de données de validation

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  IHC staining of human lung cancer using 83662-7-RR (same clone as 83662-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 83662-7-RR (same clone as 83662-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83662-7-RR (CDK9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83662-7-RR (same clone as 83662-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK9 antibody (83662-7-RR, Clone: 240600D7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83662-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83662-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83662-7-RR against Human CDK9 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83662-7-PBS cible CDK9 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDK9 Protéine recombinante Ag2318
• Nom complet: cyclin-dependent kinase 9
• Masse moléculaire calculée: 372 aa, 43 kDa
• Poids moléculaire observé: 38-42 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC001968
• Symbole du gène: CDK9
• Identification du gène (NCBI): 1025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243).

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