Anticorps Polyclonal de lapin anti-CDK9

CDK9 Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

N° de cat : CL647-11705

Synonymes

Cyclin-dependent kinase 9, CTK1, Cell division cycle 2-like protein kinase 4, CDC2L4, C-2K

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Galerie de données de validation

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  IF Staining of HeLa using CL647-11705

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 CDK9 antibody (CL647-11705) at dilution of 1:200.

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  FC experiment of HeLa using CL647-11705

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human CDK9 (CL647-11705) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-11705 cible CDK9 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDK9 Protéine recombinante Ag2318
• Nom complet: cyclin-dependent kinase 9
• Masse moléculaire calculée: 372 aa, 43 kDa
• Poids moléculaire observé: 43 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC001968
• Symbole du gène: CDK9
• Identification du gène (NCBI): 1025
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDK9(Cyclin-dependent kinase 9) is a member of the Cdc2-like family of kinases. Its cyclin partners are members of the family of cyclin T (T1, T2a and T2b) and cyclin K. The CDK9/cyclin T complexes appear to be involved in regulating several physiological processes. CDK9 has also been described as the kinase of the TAK complex, which is homologous to the P-TEFb complex and involved in HIV replication. In addition, CDK9 seems to have an anti-apoptotic function in monocytes, that may be related to its control over differentiation of monocytes (PMID: 12432243). CDK9 has two isoforms with the molecular mass of 42 kDa and 55 kDa, and the relative abundance of Cdk9(42kDa) and Cdk9(55kDa) changes in different cell types (PMID: 12706900, 15780980).

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