Anticorps Polyclonal de lapin anti-CDKN2AIP

CDKN2AIP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16615-1-AP

Synonymes

CARF, CDKN2A interacting protein, CDKN2AIP, Collaborator of ARF

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Galerie de données de validation

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  WB analysis using 16615-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16615-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16615-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16615-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16615-1-AP (CDKN2AIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16615-1-AP

  IP Result of anti-CDKN2AIP (IP:16615-1-AP, 4ug; Detection:16615-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of human kidney using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 16615-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16615-1-AP (CDKN2AIP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16615-1-AP

  Immunofluorescent analysis of HepG2 cells, using 16615-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules Jurkat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16615-1-AP cible CDKN2AIP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDKN2AIP Protéine recombinante Ag9907
• Nom complet: CDKN2A interacting protein
• Masse moléculaire calculée: 579 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC022270
• Symbole du gène: CDKN2AIP
• Identification du gène (NCBI): 55602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Cell Biosci; CDKN2AIP is critical for spermiogenesis and germ cell development; Authors - Yuming Cao
human	WB	Clin Cosmet Investig Dermatol; Genome Wide Differential Expression Profiles in Nevus Sebaceous Uncovered Low Expression of CDKN2AIP and Construction of a ceRNA Network.; Authors - Xianhong Yang