Anticorps Polyclonal de lapin anti-CDR2

CDR2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 11611-2-AP

Synonymes

CDR2, CDR62, PCD17, Yo

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Galerie de données de validation

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  WB analysis of human brain using 11611-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11611-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 11611-2-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11611-2-AP (CDR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11611-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11611-2-AP (CDR2 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules Y79
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11611-2-AP cible CDR2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDR2 Protéine recombinante Ag2183
• Nom complet: cerebellar degeneration-related protein 2, 62kDa
• Masse moléculaire calculée: 454 aa, 52 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC017503
• Symbole du gène: CDR2
• Identification du gène (NCBI): 1039
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Patients with paraneoplastic cerebellar degeneration (PCD) carry a characteristic antibody called anti-Yo. On Western blot analysis of Purkinje cells and tumor tissue from CD patient, the anti-Yo sera react with at least 2 antigens, a major species of 62 kD called CDR62 or CDR2 [PMID:18045792]. CDR2 is partly characterized, as CDR2 through its leucine zipper motif has been demonstrated to interact with c-myc, with cell cycle-related proteins and with a protein kinase, indicating that CDR2 is involved in signal transduction and gene transcription. Furthermore, CDR2 has been found to attenuate hypoxic response in renal cell carcinoma, and recent data suggest a role for CDR2 and c-myc in mitosis in cycling cells [PMID:21080165].

Publications

Species	Application	Title
human	IP, WB	Acta Neuropathol; Paraneoplastic CDR2 and CDR2L antibodies affect Purkinje cell calcium homeostasis.; Authors - Manja Schubert

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

anwesh (Verified Customer) (11-11-2019)

CDR2 was successfully IP'ed from A549 cells. The antibody had not background bands.

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: A594 cells

FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size and no additional background bands were observed around the size range. Perfect!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, U2OS, HaCaT

CDR2 Antibody Western Blot, validation (1:1000 dilution) in 293T, U2OS, HaCaT (Cat no:11611-2-AP)