Anticorps Polyclonal de lapin anti-CDX2

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22101-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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  WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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  WB analysis using 22101-1-AP (same clone as 22101-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22101-1-AP (CDX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 22101-1-AP (same clone as 22101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 22101-1-AP (same clone as 22101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 22101-1-AP (same clone as 22101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 22101-1-AP (same clone as 22101-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22101-1-AP (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 22101-1-AP (same clone as 22101-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (22101-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 22101-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22101-1-PBS cible CDX2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDX2 Protéine recombinante Ag17310
• Nom complet: caudal type homeobox 2
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 33-40 kDa
• Numéro d’acquisition GenBank: BC014461
• Symbole du gène: CDX2
• Identification du gène (NCBI): 1045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

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