Anticorps Polyclonal de lapin anti-CDX2

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55286-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of COLO 320 using 55286-1-AP (same clone as 55286-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55286-1-AP (CDX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55286-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 55286-1-AP (same clone as 55286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55286-1-AP (CDX2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55286-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 55286-1-AP (same clone as 55286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 55286-1-AP (CDX2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55286-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 55286-1-AP (same clone as 55286-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (55286-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 55286-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55286-1-PBS cible CDX2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caudal type homeobox 2
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: NM_001265
• Symbole du gène: CDX2
• Identification du gène (NCBI): 1045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

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