Anticorps Monoclonal anti-CDX2

• Phare
• Validé par KD/KO

CDX2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5A4E6

N° de cat : 60243-1-PBS

Synonymes

5A4E6, Caudal type homeobox 2, Caudal type homeobox protein 2, Caudal-type homeobox protein 2, CDX 3

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Galerie de données de validation

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  WB analysis of COLO 320 using 60243-1-Ig (same clone as 60243-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  WB analysis of SW 1990 using 60243-1-Ig (same clone as 60243-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  WB analysis of pig colon using 60243-1-Ig (same clone as 60243-1-PBS)

  pig colon tissue were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 60243-1-Ig (same clone as 60243-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60243-1-Ig (CDX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 60243-1-Ig (same clone as 60243-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (60243-1-Ig, Clone: 5A4E6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60243-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50121-1

  Cytometric bead array standard curve of MP50121-1, CDX2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60243-1-PBS. Detection antibody: 60243-3-PBS. Standard:Ag17644. Range: 0.098-100 ng/mL.

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Informations sur le produit

60243-1-PBS cible CDX2 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CDX2 Protéine recombinante Ag17644
• Nom complet: caudal type homeobox 2
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 33-40 kDa
• Numéro d’acquisition GenBank: BC014461
• Symbole du gène: CDX2
• Identification du gène (NCBI): 1045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

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