Anticorps Recombinant de lapin anti-CDX2

CDX2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3J1

N° de cat : 82659-1-PBS

Synonymes

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

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Galerie de données de validation

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  WB analysis using 82659-1-RR (same clone as 82659-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82659-1-RR (same clone as 82659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82659-1-RR (same clone as 82659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 82659-1-RR (same clone as 82659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 82659-1-RR (same clone as 82659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 82659-1-RR (same clone as 82659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 82659-1-RR (same clone as 82659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 82659-1-RR (same clone as 82659-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (82659-1-RR, Clone: 3J1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82659-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82659-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82659-1-RR against Human CDX2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

82659-1-PBS cible CDX2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CDX2 Protéine recombinante Ag17310
• Nom complet: caudal type homeobox 2
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 33-40 kDa
• Numéro d’acquisition GenBank: BC014461
• Symbole du gène: CDX2
• Identification du gène (NCBI): 1045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

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