Anticorps Polyclonal de lapin anti-CDYL

• Phare
• Validé par KD/KO

CDYL Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 17763-1-AP

Synonymes

CDY like, CDYL, CDYL1, chromodomain protein, Y like, Chromodomain Y like protein

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Galerie de données de validation

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  WB analysis of PC-3 using 17763-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17763-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17763-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 17763-1-AP

  IP result of anti-CDYL (IP:17763-1-AP, 4ug; Detection:17763-1-AP 1:1000) with MCF-7 cells lysate 3600 ug.

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  IHC staining of human breast cancer using 17763-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17763-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17763-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17763-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules MCF-7, tissu testiculaire de souris
• Résultats positifs en IP: cellules MCF-7,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

17763-1-AP cible CDYL dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDYL Protéine recombinante Ag12143
• Nom complet: chromodomain protein, Y-like
• Masse moléculaire calculée: 544 aa, 61 kDa
• Poids moléculaire observé: 66 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC108725
• Symbole du gène: CDYL
• Identification du gène (NCBI): 9425
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	Endocrinology; Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis.; Authors - Yinchuan Li
mouse	WB	Adv Sci (Weinh); Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons.; Authors - Zhao-Wei Sun
rat	WB	Drug Des Devel Ther; Sorafenib Attenuates Fibrotic Hepatic Injury Through Mediating Lysine Crotonylation; Authors - Xiao-Feng Chen
human	WB	Cancer Lett; CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment; Authors - Jin-Ge Kong ; KD Validated
human,mouse	WB,IHC,IF	Acta Pharmacol Sin; Chromodomain Y-like (CDYL) inhibition ameliorates acute kidney injury in mice by regulating tubular pyroptosis; Authors - Ting Xiang
human	WB,IF	Cell Rep; The chromodomain protein CDYL confers forebrain identity to human cortical organoids by inhibiting neuronatin; Authors - Yaming Yang ; KO Validated

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