Anticorps Monoclonal anti-CEA/CD66e

CEA/CD66e Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2B8H6

N° de cat : 68377-1-PBS

Synonymes

CEA, 2B8H6, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, CD66e

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Galerie de données de validation

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  WB analysis using 68377-1-Ig (same clone as 68377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  WB analysis using 68377-1-Ig (same clone as 68377-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68377-1-Ig (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEACAM5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 68377-1-Ig (same clone as 68377-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68377-1-Ig (CEA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68377-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68377-1-PBS cible CEA/CD66e dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CEA/CD66e Protéine recombinante Eg0247
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 5
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC034671
• Symbole du gène: CEA
• Identification du gène (NCBI): 1048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

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