Anticorps Polyclonal de lapin anti-CEA/CD66e

• Phare
• Validé par KD/KO

CEA/CD66e Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10421-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 10421-1-AP (same clone as 10421-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 10421-1-AP (same clone as 10421-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10421-1-AP (CEA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 10421-1-AP (same clone as 10421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10421-1-AP (CEA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 10421-1-AP (same clone as 10421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10421-1-AP (CEA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10421-1-AP (same clone as 10421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10421-1-AP (CEA antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10421-1-AP (same clone as 10421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10421-1-AP (CEA antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 10421-1-AP (same clone as 10421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10421-1-AP (CEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 10421-1-AP (same clone as 10421-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CEA antibody (10421-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10421-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

10421-1-PBS cible CEA/CD66e dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEA/CD66e Protéine recombinante Ag0672
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 5
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 180-200 kDa, 77 kDa
• Numéro d’acquisition GenBank: BC034671
• Symbole du gène: CEA
• Identification du gène (NCBI): 1048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis.

{{ptg:RelatedPrimaryAntibodies}}