Anticorps Polyclonal de lapin anti-CEACAM-1/CD66a

CEACAM-1/CD66a Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 32395-1-AP

Synonymes

Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1

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Galerie de données de validation

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  WB analysis using 32395-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 32395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 32395-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 32395-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CEACAM-1/CD66a antibody (32395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, intestin grêle de souris, tissu hépatique de souris
• Résultats positifs en IF-P: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32395-1-AP cible CEACAM-1/CD66a dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 100-120 kDa
• Symbole du gène: Ceacam1
• Identification du gène (NCBI): 26365
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

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