Anticorps Recombinant de lapin anti-CEACAM1/CD66a

CEACAM1/CD66a Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

241856H5

N° de cat : 84409-4-RR

Synonymes

241856H5, Bgp, Bgp1, BGP-1, Biliary glycoprotein 1

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Galerie de données de validation

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  WB analysis using 84409-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84409-4-RR (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84409-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84409-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 84409-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 84409-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84409-4-RR (CEACAM1/CD66a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 84409-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CEACAM1/CD66a antibody (84409-4-RR, Clone: 241856H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 84409-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using CEACAM1/CD66a antibody (84409-4-RR, Clone: 241856H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84409-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84409-4-RR against Mouse CEACAM1/CD66a were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu hépatique de souris, tissu d'intestin grêle de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'intestin grêle de souris, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84409-4-RR cible CEACAM1/CD66a dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CEACAM1/CD66a Protéine recombinante Eg1423
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 120 kDa
• Symbole du gène: Ceacam1
• Identification du gène (NCBI): 26365
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

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