Anticorps Monoclonal anti-CEACAM1/CD66a

CEACAM1/CD66a Monoclonal Antibody for WB, IF/ICC, FC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D1H1

N° de cat : 68653-1-Ig

Synonymes

CEACAM1, Biliary glycoprotein 1, BGPI, BGP-1, BGP1

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Galerie de données de validation

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  WB analysis using 68653-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68653-1-Ig (CEACAM1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HT-29 using 68653-1-Ig

  Immunofluorescent analysis of unfixed HT-29 cells using CEACAM1/CD66a antibody (68653-1-Ig, Clone: 1D1H1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HT-29 using 68653-1-Ig

  Immunofluorescent analysis of unfixed HT-29 cells using CEACAM1/CD66a antibody (68653-1-Ig, Clone: 1D1H1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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  FC experiment of HT-29 using 68653-1-Ig

  1x10^6 HT-29 cells were surface stained with 0.2 μg CEACAM1/CD66a Monoclonal antibody (68653-1-Ig, Clone:1D1H1) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3) was parallel stained as control. Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HT-29
• Résultats positifs en IF/ICC: cellules HT-29,
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68653-1-Ig cible CEACAM1/CD66a dans les applications de WB, IF/ICC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
• Masse moléculaire calculée: 526 aa, 58 kDa
• Poids moléculaire observé: 120-150 kDa
• Numéro d’acquisition GenBank: BC014473
• Symbole du gène: CEACAM1
• Identification du gène (NCBI): 634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

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