Anticorps Recombinant de lapin anti-CEACAM1/CD66a

CEACAM1/CD66a Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240497B4

N° de cat : 83524-3-PBS

Synonymes

CEACAM1, 240497B4, BGP, BGP1, BGP-1

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Galerie de données de validation

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  WB analysis using 83524-3-RR (same clone as 83524-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83524-3-RR (same clone as 83524-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83524-3-RR (same clone as 83524-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83524-3-RR (CEACAM1/CD66a antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83524-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83524-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83524-3-RR against Human CEACAM1 were performed. The affinity constant is 3.58 nM.

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Informations sur le produit

83524-3-PBS cible CEACAM1/CD66a dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CEACAM1/CD66a Protéine recombinante Eg0314
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
• Masse moléculaire calculée: 526 aa, 58 kDa
• Poids moléculaire observé: 135-150 kDa
• Numéro d’acquisition GenBank: BC014473
• Symbole du gène: CEACAM1
• Identification du gène (NCBI): 634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

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