Anticorps Recombinant de lapin anti-CEA/CD66e

CEA/CD66e Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240666B12

N° de cat : 83653-5-PBS

Synonymes

CEA, CEACAM5, CD66e, Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen

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Galerie de données de validation

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  WB analysis of MCF-7 using 83653-5-RR (same clone as 83653-5-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

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  WB analysis of human saliva using 83653-5-RR (same clone as 83653-5-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 83653-5-RR (CEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83653-5-RR (same clone as 83653-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83653-5-RR (same clone as 83653-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83653-5-RR (CEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 83653-5-RR (same clone as 83653-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CEA antibody (83653-5-RR, Clone: 240666B12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83653-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83653-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83653-5-RR against Human CEA were performed. The affinity constant is 0.734 nM.

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Informations sur le produit

83653-5-PBS cible CEA/CD66e dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 5
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_004363
• Symbole du gène: CEA
• Identification du gène (NCBI): 1048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. This antibody is raised against 35-685 aa of human CEA/CD66e and can cross-react with CEACAM1/CD66a and CEACAM3/CD66d.

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