Anticorps Recombinant de lapin anti-CEACAM8/CD66b

CEACAM8/CD66b Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242954B2

N° de cat : 83105-6-RR

Synonymes

CD66b, CEACAM8, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD 66b

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Galerie de données de validation

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  WB analysis using 83105-6-RR

  human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83105-6-RR

  human saliva were subjected to SDS PAGE followed by western blot with 83105-6-RR (CEACAM8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected CHO-K1 using 83105-6-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 cells using CEACAM8 antibody (83105-6-RR, Clone: 242954B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83105-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83105-6-RR against Human CEACAM8 were performed. The affinity constant is 14.1 nM.

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Applications testées

• Résultats positifs en WB: salive humain, leucocyte de sang périphérique humain
• Résultats positifs en IF/ICC: Transfected CHO-K1,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83105-6-RR cible CEACAM8/CD66b dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CEACAM8/CD66b Protéine recombinante Eg32133
• Nom complet: carcinoembryonic antigen-related cell adhesion molecule 8
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 95-100kDa
• Numéro d’acquisition GenBank: BC026263
• Symbole du gène: CEACAM8
• Identification du gène (NCBI): 1088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, CGM6 or NCA-95, is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAM8 is a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on granulocytes (PMID: 8104998; 18056392). It is involved in regulating adhesion and activation of eosinophils (PMID: 18056392).

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