Anticorps Polyclonal de lapin anti-CEBP Alpha/CEBPA

CEBP Alpha/CEBPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 29388-1-AP

Synonymes

CEBP alpha, CEBPA, C/EBP alpha, C/EBPα, CCAAT/enhancer-binding protein alpha

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Galerie de données de validation

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  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBP Alpha/CEBPA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29388-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29388-1-AP (CEBP Alpha/CEBPA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 29388-1-AP

  IP result of anti-CEBP Alpha/CEBPA (IP:29388-1-AP, 4ug; Detection:29388-1-AP 1:4000) with THP-1 cells lysate 1520 ug.

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  IHC staining of human colon using 29388-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29388-1-AP (CEBP Alpha/CEBPA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 29388-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CEBP Alpha/CEBPA antibody (29388-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules BGC-823, cellules HL-60, cellules K-562, cellules L02, cellules LNCaP, cellules THP-1, cellules U-937
• Résultats positifs en IP: cellules THP-1,
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

29388-1-AP cible CEBP Alpha/CEBPA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEBP Alpha/CEBPA Protéine recombinante Ag29947
• Nom complet: CCAAT/enhancer binding protein (C/EBP), alpha
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_004364
• Symbole du gène: CEBPA
• Identification du gène (NCBI): 1050
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc. The calcualted molecular weight of CEBPA is 38 kDa, but modified CEBPA is about 42 kDa (PMID: 19623175).

Publications

Species	Application	Title
mouse	WB	J Agric Food Chem; Enzymatic Synthesis and Evaluation of Eight Methylated Quercetin Products: In Vitro Chemical Properties and Adipogenesis Regulation; Authors - Beijun Ang
rat,human	WB	Front Pharmacol; Bushen Huoxue formula attenuates lipid accumulation evoking excessive autophagy in premature ovarian insufficiency rats and palmitic acid-challenged KGN cells by modulating lipid metabolism; Authors - Tian Li
	WB	J Virol; Dengue virus NS1 leads to downregulation of HNF4 alpha in liver cells resulting in a decrease in coagulation factors I, V, X, and XIII, contributing to coagulopathy; Authors - Sandeepan Das
mouse	WB	Toxicol Lett; The Effects of Citalopram and Sertraline on Adipogenesis and Lipogenesis in 3T3-L1 Cells; Authors - Deniz Bozdag
mouse	WB,IF	Mol Metab; Adipocyte Septin-7 attenuates obesogenic adipogenesis and promotes lipolysis to prevent obesity; Authors - Liran Xu
human	WB,IF	Int J Biol Macromol; CEBPA as a potential hub gene for cutaneous inflammation in type 2 diabetes mellitus; Authors - Mingzheng Han

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