Anticorps Recombinant de lapin anti-CEBP Alpha/CEBPA

CEBP Alpha/CEBPA Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242101C9

N° de cat : 84668-4-PBS

Synonymes

CEBP alpha, CEBPA, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα

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Galerie de données de validation

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  IF Staining of HeLa using 84668-4-RR (same clone as 84668-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84668-4-RR (same clone as 84668-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84668-4-RR (same clone as 84668-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBP Alpha/CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84668-4-RR (same clone as 84668-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR, Clone:242101C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 84668-4-RR (same clone as 84668-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CEBP Alpha/CEBPA Recombinant antibody (84668-4-RR, Clone:242101C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84668-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84668-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84668-4-RR against Human CEBP Alpha/CEBPA were performed. The affinity constant is 1.85 nM.

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Informations sur le produit

84668-4-PBS cible CEBP Alpha/CEBPA dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CEBP Alpha/CEBPA Protéine recombinante Ag29947
• Nom complet: CCAAT/enhancer binding protein (C/EBP), alpha
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_004364
• Symbole du gène: CEBPA
• Identification du gène (NCBI): 1050
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc.

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