Anticorps Recombinant de lapin anti-CEBPE

CEBPE Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251303B10

N° de cat : 86432-1-PBS

Synonymes

C/EBP epsilon, CCAAT/enhancer-binding protein epsilon, CRP1

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Galerie de données de validation

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  WB analysis of HL-60 using 86432-1-RR (same clone as 86432-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86432-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 86432-1-RR (same clone as 86432-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86432-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86432-1-RR (same clone as 86432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86432-1-PBS in a different storage buffer formulation.

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Informations sur le produit

86432-1-PBS cible CEBPE dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CEBPE Protéine recombinante Ag5589
• Nom complet: CCAAT/enhancer binding protein (C/EBP), epsilon
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31-38 kDa
• Numéro d’acquisition GenBank: BC035797
• Symbole du gène: CEBPE
• Identification du gène (NCBI): 1053
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CEBPE (CCAAT/enhancer binding protein epsilon), also known as C/EBP-epsilon and CRP1, is a transcription factor involved in late myeloid lineage differentiation and cellular function (PMID: 31201888). CEBPE is expressed in a stage-specific manner during myeloid differentiation and regulates transition from the promyelocyte to the myelocyte (PMID: 29773596). Depletion of CEBPE in Acute lymphoblastic leukaemia (ALL) cells reduces cell growth, correspondingly CEBPE binds to the promoters of electron transport and energy generation genes (PMID: 29977016).

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