Anticorps Polyclonal de lapin anti-CEBPZ

CEBPZ Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 25612-1-AP

Synonymes

CBF, CBF2, CCAAT binding factor, CEBPZ, HSP CBF, NOC1

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Galerie de données de validation

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  WB analysis of HeLa using 25612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 25612-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25612-1-AP (CEBPZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, tissu testiculaire de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

25612-1-AP cible CEBPZ dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEBPZ Protéine recombinante Ag11003
• Nom complet: CCAAT/enhancer binding protein (C/EBP), zeta
• Masse moléculaire calculée: 1054 aa, 121 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC034475
• Symbole du gène: CEBPZ
• Identification du gène (NCBI): 10153
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEBPZ (CCAAT/enhancer binding protein zeta) belongs to the CBF/Mak21 family, it plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner.

Publications

Species	Application	Title
human	WB	Cancer Sci; CEBPγ facilitates lamellipodia formation and cancer cell migration through CERS6 upregulation.; Authors - Hanxiao Shi
mouse	WB	Mol Cell Proteomics; Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression.; Authors - Tong Wu