Anticorps Polyclonal de lapin anti-CEL

• Phare
• Validé par KD/KO

CEL Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15384-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human liver using 15384-1-AP (same clone as 15384-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 15384-1-AP (same clone as 15384-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 15384-1-AP (same clone as 15384-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15384-1-AP (same clone as 15384-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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  IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), INS antibody (66198-1-Ig, Clone: 4B6A7, red). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15384-1-AP (same clone as 15384-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15384-1-AP (CEL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15384-1-PBS cible CEL dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEL Protéine recombinante Ag5262
• Nom complet: carboxyl ester lipase (bile salt-stimulated lipase)
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC042510
• Symbole du gène: CEL
• Identification du gène (NCBI): 1056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.

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