Anticorps Recombinant de lapin anti-CEL

CEL Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242761B11

N° de cat : 85081-4-PBS

Synonymes

Bile salt-activated lipase, 242761B11, BAL, Bile salt-stimulated lipase, BSDL

---

Galerie de données de validation

• 

  WB analysis using 85081-4-RR (same clone as 85081-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85081-4-RR (CEL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85081-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 85081-4-RR (same clone as 85081-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85081-4-RR (CEL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85081-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 85081-4-RR (same clone as 85081-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85081-4-RR (CEL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85081-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85081-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85081-4-RR against Human CEL were performed. The affinity constant is 2.46 nM.

---

Informations sur le produit

85081-4-PBS cible CEL dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CEL Protéine recombinante Ag5262
• Nom complet: carboxyl ester lipase (bile salt-stimulated lipase)
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC042510
• Symbole du gène: CEL
• Identification du gène (NCBI): 1056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.

{{ptg:RelatedPrimaryAntibodies}}