Anticorps Polyclonal de lapin anti-CEND1

CEND1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 13280-1-AP

Synonymes

BM88 antigen, BM88

---

Galerie de données de validation

• 

  WB analysis using 13280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13280-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 13280-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 13280-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CEND1 antibody (13280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

13280-1-AP cible CEND1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEND1 Protéine recombinante Ag4024
• Nom complet: cell cycle exit and neuronal differentiation 1
• Masse moléculaire calculée: 149 aa, 15 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC034732
• Symbole du gène: CEND1
• Identification du gène (NCBI): 51286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell cycle exit and neuronal differentiation 1 (CEND1) also known as BM88, is a neuronal protein associated in vivo with terminal neuron-generating divisions, marking the exit of proliferative cells from the cell cycle (PMID: 17971443). CEND1 expression is critical for the NEUROG2-driven reprogramming of astrocytes, suggesting the existence of a reciprocal feedback loop leading to neurogenesis (PMID: 26321141). CEND1 is an integral membrane protein composed of two 23-kDa polypeptide chains linked together by disulfide bridges.

Publications

Species	Application	Title
goat	WB	Cell Death Discov; Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers; Authors - Zhimin Wang
rat	WB	J Ethnopharmacol; The Protective Effects and Mechanisms of Essential oil from Chimonanthus nitens Oliv. Leaves in Allergic Rhinitis Based on the NF-κB and T-bet/GATA-3 signaling pathways; Authors - Xiaoying Huang

{{ptg:RelatedPrimaryAntibodies}}